Coding
yCCS

Part:BBa_K380008:Design

Designed by: Andreas Constantinou, Emmelie Lidh, Nina Schiller, Johan Nordholm   Group: iGEM10_Stockholm   (2010-10-25)

Yeast copper chaperon protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 250


Design Notes

The start and stop codon was removed and the gene was redesigned with BioBrick prefix and suffix according to assembly standard 25 using specifically designed primers and PCR. A restriction site for EcoRI at basepair number 228 was removed using site-directed mutagenesis PCR.


Source

Saccharomyces cerevisiae S288c Ccs1p (CCS1), mRNA ([http://www.ncbi.nlm.nih.gov/nuccore/NM_001182535.1?report=genbank&log$=seqview GeneBank: NM_001182535.1]), 750 nucleotide gene. First identified and characterized by [http://www.ncbi.nlm.nih.gov/pubmed/9295278 Culotta et al. (1997)].

References